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Product‐specific sample clean‐up and HPLC analysis of aflatoxins for a dietary product
Author(s) -
Zahn Michael,
Jeong Mijeong Lee,
Wang Desheng,
Trinh Thao,
Fay Brooke,
Ma Wenwen
Publication year - 2009
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.1132
Subject(s) - aflatoxin , chemistry , chromatography , high performance liquid chromatography , sample preparation , ingredient , clean up , scutellaria baicalensis , food science , extraction (chemistry) , medicine , alternative medicine , traditional chinese medicine , pathology
A sample clean‐up procedure has been developed for a complex dietary ingredient, Unigen Inc. (Lacey, WA, USA) product A (a proprietary mixture of Scutellaria baicalensis and Acacia catechu extracts), for the determination of aflatoxins by HPLC. Objective To develop an appropriate sample specific clean‐up procedure that removes interferences from the sample for the determination of total aflatoxins B 1 , B 2 , G 1 and G 2 at or lower than 20 ppb by HPLC. Methodology The sample specific clean‐up procedure was developed based on the modification of AOAC method 990.33. Results Coextract interferences were removed with the clean‐up procedure. The recovery of total aflatoxins was 70% with RSD about 2%; the LOD and LOQ were 1.6 and 5.2 ppb, respectively, for total aflatoxins; the linearity R 2 > 0.99. None of the samples tested showed the presence of detectable aflatoxins. Conclusion The modified clean‐up method was effective to remove coextract interferences from Unigen product A samples. Copyright © 2009 John Wiley & Sons, Ltd.