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Fingerprint of Hedyotis diffusa Willd. by HPLC‐MS
Author(s) -
Yang Ting,
Yang YiHua,
Yang JuYun,
Chen BenMei,
Duan JuPing,
Yu ShuYi,
Ouyang HongTao,
Cheng JunPing,
Chen YuXiang
Publication year - 2008
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.1071
Subject(s) - chemistry , chromatography , high performance liquid chromatography , ursolic acid , ferulic acid , fingerprint (computing) , oleanolic acid , acetic acid , chemical constituents , biochemistry , artificial intelligence , medicine , alternative medicine , pathology , computer science
A HPLC‐MS fingerprint method has been developed based on the consistent chromatographic features of the major chemical constituents among 10 batches of Hedyotis diffusa Willd. Chromatographic separation was conducted on a Hypersil‐Keystone Hypurity C 18 column using methanol:water:acetic acid as the mobile phase. Major compounds, including oleanolic acid, ursolic acid and ferulic acid, were analysed by HPLC‐MS. Their analysis was ascertained by comparison with data derived from the standard compounds. The HPLC‐MS fingerprint was successfully applied to analyse and differentiate samples from different geographical origins, or processing methods. H . diffusa was well distinguished from Hedyotis chrysotricha by HPLC‐MS. Therefore the establishment of fingerprint of H . diffusa is critical in assessing and controlling its overall quality. Copyright © 2008 John Wiley & Sons, Ltd.