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A survey of sesamin and composition of tocopherol variability from seeds of eleven diverse sesame ( Sesamum indicum L.) genotypes using HPLC‐PAD‐ECD
Author(s) -
Williamson Kelly S.,
Morris J. Brad,
Pye Quentin N.,
Kamat Chandrashekhar D.,
Hensley Kenneth
Publication year - 2008
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.1050
Subject(s) - sesamin , sesamum , tocopherol , chemistry , high performance liquid chromatography , lignan , food science , sesame seed , horticulture , chromatography , biology , vitamin e , raw material , antioxidant , biochemistry , organic chemistry , stereochemistry
The objective of this study was to determine the composition and content of sesamin and desmethyl tocopherols such as α ‐tocopherol ( α T), δ ‐tocopherol ( δ T) and γ ‐tocopherol ( γ T) in seeds of sesame ( Sesamum indicum L.) for 11 genotypes conserved in the United States Department of Agriculture (USDA), Agricultural Research Service (ARS) and Plant Genetic Resources Conservation Unit (PGRCU) in Griffin, Georgia, USA. Seed accessions studied were collections from eight countries worldwide, including one landrace from Thailand and two cultivars from Texas, USA. Novel methodologies and analytical techniques described herein consisted of reverse‐phase high‐performance liquid chromatography (HPLC) connected in series with two detection systems specific for each analyte class. Photodiode array detection was employed for sesamin analysis and electrochemical array detection was used in the determination of tocopherols. A preliminary study was conducted to assess sesamin levels in 2003 and tocopherol levels in 2004 from sesame seed samples conserved at the USDA, ARS and PGRCU. In 2005, sesame seed samples were grown, harvested and evaluated for sesamin as well as tocopherol levels. The overall results ( n = 3) showed that sesamin, α T, δ T and γ T levels were 0.67–6.35 mg/g, 0.034–0.175 µg/g, 0.44–3.05 µg/g and 56.9–99.3 µg/g respectively, indicating that the sesame seed accessions contained higher levels of sesamin and γ T compared with α T and δ T. Statistical analysis was conducted and significant differences were observed among the 11 different sesame genotypes. This suggests that genetic, environmental and geographical factors influence sesamin and desmethyl tocopherol content. Copyright © 2007 John Wiley & Sons, Ltd.

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