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High‐performance liquid chromatography–electrospray mass spectrometry for the simultaneous determination of multiple active components in Sheng‐Mai San, a Prescription of traditional Chinese medicine
Author(s) -
Chang YiWei,
Yao HsienTsung,
Chien DuShieng,
Yeh TengKuang
Publication year - 2007
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.1046
Subject(s) - chemistry , chromatography , analyte , mass spectrometry , electrospray , gradient elution , high performance liquid chromatography , detection limit , elution , selected reaction monitoring , analytical chemistry (journal) , tandem mass spectrometry
An advanced and reliable HPLC‐MS method was developed for the simultaneous quantification of eight active components (ginsenosides Rf, Rg 2 , Rg 3 , Rh 1 and Rh 2 , gomisin A, methylophioponanone B and schizandrin) in Sheng‐Mai San, a traditional Chinese medicine. The elution of multiple components was performed using a C 18 column with stepwise gradient elution. The detection of individual analytes was monitored by electrospray MS scanning from 300 to 1000 m/z in the positive ion mode, with the limits of detection of these components ranging from 0.06 to 1 µg/mL at a signal‐to‐noise ratio of ≥5. The intra‐ and inter‐day accuracies ranged from 95.1 to 104.4%, and the overall precision was less than 9.3%. The recoveries of the analytes were ≥96.6%. The method was validated and found suitable for the determination of active components present in Sheng‐Mai San preparation. Copyright © 2007 John Wiley & Sons, Ltd.