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Separation and free radical‐scavenging activity of major curcuminoids of Curcuma longa using HPTLC‐DPPH method
Author(s) -
Pozharitskaya Olga N.,
Ivanova Svetlana A.,
Shikov Alexander N.,
Makarov Valery G.
Publication year - 2007
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.1035
Subject(s) - chemistry , curcuma , zingiberaceae , dpph , curcumin , chromatography , ascorbic acid , rhizome , scavenging , antioxidant , organic chemistry , traditional medicine , food science , biochemistry , medicine
A direct HPTLC assay was developed for the determination of total curcuminoids and three individual curcuminoids, curcumin, demethoxycurcumin and bisdemethoxycurcumin. In addition, a new procedure was developed to separate and quantitative the free radical‐scavenging activity of individual compounds from the rhizome of Curcuma longa L. (Zingiberaceae) based on the combination of HPTLC with a diode array detector (DAD) and post chromatographic DPPH • radical derivatisation. It was established that both individual curcuminoids and the extract of C. longa were capable of scavenging DPPH • radicals. From the estimated ID 50 values, it can be seen that the order of activity was curcumin > demethoxycurcumin > bisdemethoxycurcumin >> ascorbic acid. However, the ID 50 values of curcuminoids were not significantly different. The data indicates the presence of a synergistic mechanism of antiradical activity of curcuminoids. Copyright © 2007 John Wiley & Sons, Ltd.