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Preparative isolation and purification of chemical components from Aconitum coreanum by high‐speed counter‐current chromatography coupled with evaporative light scattering detection
Author(s) -
Tang Qingfa,
Yang Chunhua,
Ye Wencai,
Liu Jinghan,
Zhao Shouxun
Publication year - 2007
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.1030
Subject(s) - chemistry , aconitum , countercurrent chromatography , chromatography , chromatography detector , butanol , alkaloid , high performance liquid chromatography , organic chemistry , ethanol
Abstract Aconitum coreanum (Lèvl.) Rapaics (Guanbaifu in Chinese) is a widely used, centuries‐old Chinese herb. A preparative high‐speed counter‐current chromatography (HSCCC) coupled with evaporative light scattering detection (ELSD) method was employed for isolation and purification of alkaloids from the crude extract of Aconitum coreanum (Lèvl.) Rapaics using ethyl acetate– n ‐butanol–methanol–0.2 m HCl (7:2:2:7, v/v) as a two‐phase solvent system. Six alkaloids, including GFO, GFQ, GFZ, hetisinone, hetisine and GFAA, were obtained in one‐step separation. The purity of these compounds was 97.6, 93.8, 91.8, 91.9, 96.2 and 91.1%, respectively. Copyright © 2007 John Wiley & Sons, Ltd.