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Analysis of galanthamine‐type alkaloids by capillary gas chromatography–mass spectrometry in plants
Author(s) -
Berkov Strahil,
Bastida Jaume,
Viladomat Francesc,
Codina Carles
Publication year - 2008
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.1028
Subject(s) - chemistry , chromatography , amaryllidaceae , mass spectrometry , extraction (chemistry) , selected ion monitoring , detection limit , gas chromatography–mass spectrometry , amaryllidaceae alkaloids , alkaloid , stereochemistry , botany , biology
Galanthamine, an acetylcholinesterase inhibitor used for the treatment of Alzheimer's disease, and galanthamine‐type alkaloids are synthesised in different plants of the family Amaryllidaceae. A capillary gas chromatographic–mass spectroscopic (CGC‐MS) method for the separation of 7 galanthamine type alkaloids, including galanthamine and epigalanthamine, is described in the present paper. A simple method for the routine quantification of galanthamine in plants was developed using pre‐packed columns with diatomaceous earth (Isolute HM‐N), allowing simultaneous preparation of a large number of samples. Galanthamine showed excellent linearity in the range from 50 to 1000 µg/mL and the limit of quantification was 5 µg/mL in total ion current mode and 1.6 ng/mL in selected ion monitoring mode. The recovery of galanthamine was more than 90%. Interday reproducibility (RSD) of the extraction was 2.74%. A method to find and to microextract Amaryllidaceae alkaloids in low‐mass plant samples is also described. Copyright © 2008 John Wiley & Sons, Ltd.