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Determination of camptothecins in DMSO extracts of Nothapodytes foetida by direct injection capillary electrophoresis
Author(s) -
Hsiao HsienYi,
Cheng TzongJih,
Yang GeMing,
Huang IJen,
Chen Richie L.C.
Publication year - 2007
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.1026
Subject(s) - chemistry , camptothecin , capillary electrophoresis , chromatography , chloroform , solvent , capillary action , organic chemistry , materials science , composite material
A rapid capillary electrophoresis procedure was developed for determining the anti‐cancer components, camptothecins, in Nothapodytes foetida. The hydrophobic compound was extracted from plant tissue (ca. 1 mL of DMSO for 100 mg of dried plant tissue) with a water‐miscible organic solvent, DMSO, at elevated temperature (60°C). The extract was directly injected into the separation capillary (untreated fused silica, 34 cm in length, 75 µm i.d.) and analysed in MEKC mode (369 nm). Within 5 min of migration, camptothecins were successfully separated and quantified by adding organic modifiers to the running buffer (20% DMSO, 90 m m SDS in 10 m m borate buffer, pH 8.60). The linear dynamic range for camptothecin was from 5 to 400 µg/mL. This method was proven to be very suitable for monitoring the amount of camptothecins during the cultivation of the medicinal plant. Copyright © 2007 John Wiley & Sons, Ltd.