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A reversed‐phase high‐performance liquid chromatographic method for the determination of aloesin, aloeresin a and anthraquinone in Aloe ferox
Author(s) -
Zahn Michael,
Trinh Thao,
Jeong Mijeong Lee,
Wang Desheng,
Abeysinghe Padmapriya,
Jia Qi,
Ma Wenwen
Publication year - 2007
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.1024
Subject(s) - chemistry , detection limit , chromatography , high performance liquid chromatography , anthraquinone , methanol , linearity , reversed phase chromatography , analytical chemistry (journal) , organic chemistry , physics , quantum mechanics
A reversed‐phase HPLC method for the quantification of aloesin, aloeresin a and anthraquinone (as barbaloin) in Aloe ferox Miller and aloe‐related products has been developed and validated. The method utilized a C 18 column with a water–methanol gradient and UV detection at 297 nm. The method validation included linearity, accuracy, precision, limit of detection, limit of quantitation, specificity and standard solution stability. The method showed good linearity ( r > 0.99 for all components) and recovery (>85% for all components). The detection and quantitation limits for barbaloin were determined to be 0.02 and 0.1 ppm at signal‐to‐noise ratios of approximately 3:1 and 10:1, respectively. Copyright © 2007 John Wiley & Sons, Ltd.