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Application of analytical and preparative high‐speed counter‐current chromatography for the separation of Z ‐ligustilide from a crude extract of Angelica sinensis
Author(s) -
Wang Xiao,
Shi Xingang,
Li Fuwei,
Liu Jianhua,
Cheng Chuange
Publication year - 2008
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.1023
Subject(s) - chemistry , angelica sinensis , countercurrent chromatography , chromatography , mass spectrometry , petroleum ether , high performance liquid chromatography , analytical chemistry (journal) , extraction (chemistry) , traditional chinese medicine , medicine , alternative medicine , pathology
Z ‐Ligustilide was separated and purified from the traditional Chinese medicinal plant Angelica sinensis by high‐speed counter‐current chromatography (HSCCC). Analytical HSCCC was first used for the systematic selection of the two‐phase solvent system. Preparative HSCCC separation was performed with a two‐phase solvent system composed of petroleum ether (60–90°C)–ethanol–water at an optimum volume ratio of 10:17:10 (v/v). A total of 38 mg Z ‐ligustilide at 98.8% purity was obtained in one step from 200 mg crude extract as determined by HPLC analysis. The structure of the target compound was identified by electron impact ionisation mass spectrometry. Copyright © 2008 John Wiley & Sons, Ltd.