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Preparative separation of four triterpene saponins from radix astragali by high‐speed counter‐current chromatography coupled with evaporative light scattering detection
Author(s) -
Peng Jinyong,
Dong Fuqiu,
Qi Yan,
Han Xu,
Xu Youwei,
Xu Lina,
Xu Qiwei,
Liu Kexin,
Zhu Zhenyu
Publication year - 2007
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.1011
Subject(s) - chemistry , triterpene , chromatography , countercurrent chromatography , astragaloside , radix (gastropod) , elution , ethyl acetate , high performance liquid chromatography , medicine , botany , alternative medicine , pathology , biology
Four triterpene saponins, including astragaloside IV, astragaloside II, astragaloside I and acetylastragaloside I, were successfully isolated and separated by high‐speed counter‐current chromatography coupled with evaporative light scattering detection from Radix Astragali using stepwise elution with a pair of solvent systems composed of n ‐hexane:ethyl acetate:ethanol:water in volume ratios of 1:0.6:0.6:1 and 1:1:1:1 (by volume). The isolation produced 26.5 mg astragaloside IV, 28.2 mg astragaloside II, 48.7 mg astragaloside I and 17.6 mg acetylastragaloside I with purities of 97.6, 96.4, 98.8 and 96.8%, respectively, determined by high‐performance liquid chromatography from 250 mg crude extract. The chemical structures of the isolated compounds were identified by UV, NMR and MS, and confirmed by authentic standards. Copyright © 2007 John Wiley & Sons, Ltd.