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In vitro cytotoxic effect of spikenard herbal immobilized polymer nano‐biocomposites: Toward bioinspired antimicrobial agent
Author(s) -
Priya Sahariya,
Jeyajothi Gabriel
Publication year - 2019
Publication title -
polymer composites
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.577
H-Index - 82
eISSN - 1548-0569
pISSN - 0272-8397
DOI - 10.1002/pc.25039
Subject(s) - materials science , crystallinity , nuclear chemistry , polymer , scanning electron microscope , hacat , nanocomposite , nanoparticle , chemical engineering , nanotechnology , composite material , chemistry , biochemistry , in vitro , engineering
We report the intrinsic biological properties of nickel oxide nanoparticle (NiO NPs) encapsulated Nardostachys jatamansi DC‐based polymethyl methacrylate (PMMA) via free radical polymerization technique. A simple and cost effective NiO NPs was adapted to synthesize via sol–gel/co‐precipitation method to afford crystalline structures of NPs and it was confirmed by X‐ray diffraction (XRD) patterns (2θ at 38.78 [111] and 62.66 [220]). The results demonstrated that the sparingly loaded NiO NPs onto composites exhibited the lowest crystallinity, which led to better dispersion into the polymer matrix and also observed by scanning electron microscopy (SEM) and atomic force microscopy (AFM). ATR‐IR enunciate the functional groups of polymer nano‐biocomposites attributed at 1730 cm −1 (C=O), 1446 cm −1 (CH 3 ‐asymmetric deformation), 1162 cm −1 (C‐N), and 822 cm −1 (C‐H), which were found to be good agreement for the formation of polymer composites and further confirmed their structural identification by NMR, fluorescence spectroscopy and UV– vis spectroscopy. This hybrid nano‐biocomposite has been utilized for antimicrobial studies using a gram positive and gram‐negative bacteria and showed better results on minimum bactericidal concentration (MBC) assay against Micrococcus luteus, Staphylococcus epidermidis, Yersinia enterocolitica, and Klebsiella pneumoniae . In addition, cell morphology and proliferation were monitored by fluorescence microscopy. The MTT assay results showed that the nano‐biocomposites possessed significantly low cytotoxicity against human keratinocyte cell lines (HaCaT). POLYM. COMPOS., 40:2301–2310, 2019. © 2018 Society of Plastics Engineers