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Preparation of tricalcium phosphate–calcium alginate composite flat sheet membranes and their application for protein release
Author(s) -
Li Sidi,
Kan Bohong,
Zhao Kongyin,
Ren Tong,
Lin Beibei,
Wei Junfu,
Chen Tian
Publication year - 2015
Publication title -
polymer composites
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.577
H-Index - 82
eISSN - 1548-0569
pISSN - 0272-8397
DOI - 10.1002/pc.23098
Subject(s) - materials science , aqueous solution , membrane , chemical engineering , fourier transform infrared spectroscopy , composite number , adsorption , swelling , phosphate , polymer , calcium , composite material , nuclear chemistry , chemistry , organic chemistry , metallurgy , biochemistry , engineering
Tricalcium phosphate–calcium alginate (TCP–CA) composite flat sheet membranes with the thickness of 150–200 μm were prepared by Ca 2+ crosslinking of TCP and sodium alginate (SA) aqueous solution. The composite flat sheet membranes were characterized by SEM, FTIR, DSC and BET method. The mechanical properties of the membranes were tested with a tensile testing machine in wet form. Hemoglobin (HB) was immobilized in the following four matrices (1) HB was adsorbed onto the TCP powders directly, (2) HB and SA dissolved together, and then crosslinked by CaCl 2 , (3) HB was pre‐adsorbed onto TCP powders, and then mixed with SA aqueous solution, finally crosslinked by CaCl 2 , (4) HB and TCP powders were individually dispersed in the SA aqueous solution and then crosslinked by CaCl 2 . The release behaviors of the four materials were investigated in phosphate buffer (pH = 6.86), Tris‐HCl solution (pH = 7.4) and NaCl solution (0.9 wt%). SEM observation showed that the TCP particles were well embedded and homogeneously distributed in the alginate matrix. DSC and FTIR indicated that there were additional interactions between the TCP and the polymer. TCP can improve the mechanical properties of CA membranes and the swelling ratio of TCP–CA membrane in NaCl solution decreased with the increase of TCP content. The sustained release behaviors of matrix 3 were shown in PBS, Tris‐HCl and NaCl solutions. POLYM. COMPOS., 36:1899–1906, 2015. © 2014 Society of Plastics Engineers