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Stability of β‐galactosidase immobilized on composite microspheres of artemisia seed gum and chitosan
Author(s) -
Zhang Ji,
Zhang Shengtang,
Wang Yunpu,
Gao Sufang
Publication year - 2008
Publication title -
polymer composites
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.577
H-Index - 82
eISSN - 1548-0569
pISSN - 0272-8397
DOI - 10.1002/pc.20337
Subject(s) - glutaraldehyde , chitosan , composite number , thermal stability , immobilized enzyme , materials science , microsphere , natural gum , substrate (aquarium) , chemical engineering , enzyme , chemistry , chromatography , composite material , organic chemistry , polysaccharide , biology , ecology , engineering
In this work, composite microspheres were prepared by using artemisia seed gum and chitosan as a source. The composite microspheres have activated aldehyde groups by using glutaraldehyde. β‐Galactosidase was covalently bound on these activated microspheres. The properties of the immobilized enzyme were investigated and compared with those of the free enzyme, for which o ‐nitrophenol β‐ D ‐galactopyranoside (ONPG) was chosen as a substrate. The results showed that the pH and thermal stability of the immobilized β‐galactosidase were higher than those of the soluble one. Apart from these, the Michaelis constant K m was evaluated for the immobilized β‐galactosidase and the soluble enzyme. The immobilized β‐galactosidase exhibited better environmental adaptability and reusability than the soluble one. POLYM. COMPOS., 29:9–14, 2008. © 2007 Society of Plastics Engineers