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Donor characteristics and intraoperative total nucleated cell count influence hematopoietic progenitor cell yield of healthy donor bone marrow grafts
Author(s) -
Kalin Jacob,
Nguyen Anh Thy H.,
Oshrine Benjamin
Publication year - 2021
Publication title -
pediatric blood and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.116
H-Index - 105
eISSN - 1545-5017
pISSN - 1545-5009
DOI - 10.1002/pbc.28902
Subject(s) - medicine , bone marrow , cd34 , haematopoiesis , hematopoietic cell , transplantation , urology , progenitor cell , hematopoietic stem cell transplantation , surgery , nuclear medicine , andrology , stem cell , biology , genetics
Background Bone marrow graft cell content impacts engraftment potential after allogeneic hematopoietic cell transplantation (alloHCT). Surrogates, such as intraoperative total nucleated cell count (ioTNC), are of unclear utility in predicting final graft characteristics. In addition, demographic and clinical factors may influence graft cellular profile and recipient engraftment. Procedure We retrospectively reviewed marrow harvests at our institution performed between 2009 and 2019. During this time, an ioTNC was measured after 50% of the projected final graft volume was collected. Regression models were used to assess associations between ioTNC (cells/µL) and final graft CD34+ (cells/mL), and between graft and donor characteristics and final graft CD34+ (cells/mL). Results Fifty‐three marrow harvests and donor‐recipient pairs were analyzed. Median (range) donor and recipient ages were 13 (0.7‐28) years and 9 (0.2‐21) years, respectively. The median ratio of donor/recipient weight was 1.225 kg (range 0.31‐7.13). Median total volume of harvested marrow was 15.3 mL/kg (range 4.3‐20.4) of donor weight and 19.4 mL/kg (range 4.7‐87.4) of recipient weight. Median ioTNC was 20 930/µL (range 6600‐44310) or 2.1 × 10 9 /mL, corresponding to median predicted final graft TNC of 3.59 × 10 8 /kg recipient weight (range 1.28‐19.42 × 10 8 ). Simple linear regression between ioTNC and CD34+ cells/mL resulted in an R 2 of 0.42. Least absolute shrinkage and selection operator (LASSO) regression produced a moderately predictive model consisting of ioTNC, donor age, and donor weight (adjusted R 2 = 0.7) of final graft CD34+ cells/mL. Conclusions ioTNC and certain donor characteristics correlate moderately well with marrow product CD34+ cells/mL, potentially informing donor selection and marrow procurement strategies.