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Very long‐term molecular follow‐up of minimal residual disease in patients with neuroblastoma
Author(s) -
Tchirkov Andrei,
Greze Victoria,
Plantaz Dominique,
Rouel Nadège,
Vago Philippe,
Kanold Justyna
Publication year - 2018
Publication title -
pediatric blood and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.116
H-Index - 105
eISSN - 1545-5017
pISSN - 1545-5009
DOI - 10.1002/pbc.27404
Subject(s) - neuroblastoma , minimal residual disease , medicine , oncology , transplantation , polymerase chain reaction , reverse transcriptase , chemotherapy , disease , stem cell , immunology , cancer research , cell culture , gene , bone marrow , biology , genetics
Abstract In high‐risk neuroblastoma (HR‐NB), the clinical significance of long‐term minimal residual disease (MRD) monitoring using quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) for neuroblastoma mRNAs has not been investigated. We report long‐term MRD follow‐ups of four patients with HR‐NB throughout the disease (diagnosis, remission, and relapse) and treatment course (chemotherapy, autologous and allogeneic stem cell transplantation, and donor lymphocyte and natural killer cell infusions). The results showed the stability of mRNA marker expression after different treatments and demonstrated their validity to predict relapse and assess therapeutic response. This opens up the possibility of investigating the utility of long‐term molecular monitoring of MRD in prospective multicenter studies.