Premium
Overexpression of minichromosome maintenance protein 10 in medulloblastoma and its clinical implications
Author(s) -
Senfter Daniel,
Erkan Erdogan Pekcan,
Özer Erdener,
Jungwirth Gerhard,
Madlener Sibylle,
Kool Marcel,
Ströbel Thomas,
Saydam Nurten,
Saydam Okay
Publication year - 2017
Publication title -
pediatric blood and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.116
H-Index - 105
eISSN - 1545-5017
pISSN - 1545-5009
DOI - 10.1002/pbc.26670
Subject(s) - medulloblastoma , rna interference , gene silencing , minichromosome maintenance , cancer research , cell growth , cell culture , minichromosome , biology , microbiology and biotechnology , cell , gene expression profiling , gene expression , gene , cell cycle , genetics , rna , control of chromosome duplication , genome
Background Overexpression of minichromosome maintenance (MCM) proteins 2, 3, and 7 is associated with migration and invasion in medulloblastoma (MB). However, expression profiling of all prereplication complex (pre‐RC) has not been addressed in MBs. Procedure We performed mRNA expression profiling of a large set of pre‐RC elements in cell lines and tumor tissues of MB. RNAi technology was employed for functional studies in MB cell lines. Results Our data showed that most of the pre‐RC components are significantly overexpressed in MB. Among all pre‐RC mRNAs, MCM10 showed the highest level of expression (∼500‐ to 1,000‐fold) in MB cell lines and tissues compared to the levels detected in cerebellum. In addition, RNAi silencing of MCM10 caused reduced cell proliferation and cell viability in MB cells. Conclusions Taken together, our study reveals that the pre‐RC is dysregulated in MB. In addition, MCM10, a member of this complex, is significantly overexpressed in MB and is required for tumor cell proliferation.