Premium
In vivo detection of polyomaviruses JCV and SV40 in mesenchymal stem cells from human umbilical cords
Author(s) -
Comar Manola,
Delbue Serena,
Zanotta Nunzia,
Valencic Erica,
Piscianz Elisa,
Del Savio Rossella,
Tesser Alessandra,
Tommasini Alberto,
Ferrante Pasquale
Publication year - 2014
Publication title -
pediatric blood and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.116
H-Index - 105
eISSN - 1545-5017
pISSN - 1545-5009
DOI - 10.1002/pbc.24943
Subject(s) - merkel cell polyomavirus , bk virus , virology , jc virus , umbilical cord , mesenchymal stem cell , biology , polyomavirus infections , bone marrow , stem cell , virus , medicine , immunology , merkel cell carcinoma , progressive multifocal leukoencephalopathy , carcinoma , kidney , microbiology and biotechnology , kidney transplantation , genetics , endocrinology
Background Multipotent stromal cells are present in the Wharton's jelly matrix (WJSC) of the umbilical cord and can be used as an allogeneic source of cells to treat immunological disorders. Recently it was demonstrated that adult bone marrow (BM)‐derived mesenchimal stromal cells (MSC) are susceptible to infection with viruses showing potential oncogenic properties, such as the polyomavirus JC (JCV). The aim of this study was to investigate the presence of human polyomaviruses (JCV, BK Virus‐BKV, SV40, and Merkel cell polyomavirus‐MCPyV) in WJSC, and explore the risk of infection. Procedure MSC samples from 35 umbilical cords were investigated by quantitative Real Time PCRs for the presence of DNA sequences of JCV, BKV, SV40, and MCPyV. Results JCV DNA was detected in 1/35 (2.8%) of MSC samples, while SV40 DNA was found in 3/35 (8.6%) of the examined samples. None of the samples showed sequences of BKV and MCPyV. Conclusions The present study demonstrates the in vivo ability of polyomaviruses to infect WJSC. Since the therapeutic approach with the WJSC has high potentiality and a more intensive use can be easily hypothesized, the need to develop consensus guidelines to detect rare viral infections in MSC is pressing. Pediatr Blood Cancer 2014; 61:1347–1349. © 2014 Wiley Periodicals, Inc.