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Adoptive immunotherapy with antigen‐specific T cells during extracorporeal membrane oxygenation (ECMO) for adenovirus‐related respiratory failure in a child given haploidentical stem cell transplantation
Author(s) -
Di Nardo Matteo,
Li Pira Giuseppina,
Amodeo Antonio,
Cecchetti Corrado,
Giorda Ezio,
Ceccarelli Stefano,
Brescia Letizia P.,
Pirozzi Nicola,
Rutella Sergio,
Locatelli Franco,
Bertaina Alice
Publication year - 2014
Publication title -
pediatric blood and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.116
H-Index - 105
eISSN - 1545-5017
pISSN - 1545-5009
DOI - 10.1002/pbc.24753
Subject(s) - medicine , hematopoietic stem cell transplantation , extracorporeal membrane oxygenation , immunology , stem cell , transplantation , adoptive cell transfer , immunotherapy , t cell , antigen , immune system , biology , microbiology and biotechnology
We report on the successful infusion of human adenovirus (HAdV)‐specific T cells in a child with congenital amegakaryocytic thrombocytopenia, given T‐cell‐depleted hematopoietic stem cell transplantation (HSCT) from the HLA‐haploidentical mother during extracorporeal membrane oxygenation (ECMO) for severe HAdV‐related respiratory failure. Donor‐derived, interferon (IFN)‐γ‐secreting HAdV‐specific T cells were enriched using the cytokine capture assay, after in vitro stimulation with overlapping peptides from the immunodominant HAdV5 hexon protein. Two weeks after T‐cell transfer, viral load decreased and ECMO was discontinued. T‐cell responses to HAdV antigens were documented after four weeks and were associated with viral clearance, immune reconstitution and clinical amelioration. Pediatr Blood Cancer 2014;61:376–379. © 2013 Wiley Periodicals, Inc.