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Absence of epstein–barr and cytomegalovirus infection in neuroblastoma cells by standard detection methodologies
Author(s) -
Sehic Daniel,
Forslund Ola,
Sandén Emma,
Mengelbier Linda Holmquist,
Karlsson Jenny,
Bzhalava Davit,
Ekström Johanna,
Warenholt Janina,
Darabi Anna,
Dillner Joakim,
Øra Ingrid,
Gisselsson David
Publication year - 2013
Publication title -
pediatric blood and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.116
H-Index - 105
eISSN - 1545-5017
pISSN - 1545-5009
DOI - 10.1002/pbc.24535
Subject(s) - human cytomegalovirus , neuroblastoma , cytomegalovirus , epstein–barr virus , virology , virus , medicine , in situ hybridization , pathogenesis , rna , immune system , herpesviridae , immunohistochemistry , polymerase chain reaction , gene , biology , immunology , viral disease , gene expression , cell culture , genetics
Indications exist in the scientific literature that infection with human herpes family viruses may contribute to the pathogenesis of neuroblastoma (NB). However, systematic investigations regarding viral presence in NB cells have been scarcely reported. Here, the presence of DNA from Epstein–Barr virus (EBV) and human cytomegalovirus (HCMV) was assessed by PCR in 12 NBs, supplemented with RNA in situ hybridization, immunohistochemical detection, and high‐throughput DNA sequencing. These standard methods did not detect infection by EBV or HCMV in NB cells in any tumor, while occasional immune cells were positive for EBV RNA or HCMV protein in four cases. Pediatr Blood Cancer 2013;160:E91–E93. © 2013 Wiley Periodicals, Inc.