Premium
Use of capillary blood to diagnose hereditary spherocytosis
Author(s) -
Crisp Renée L.,
Solari Liliana,
Gammella Daniel,
Schvartzman Gabriel A.,
Rapetti María Cristina,
Donato Hugo
Publication year - 2012
Publication title -
pediatric blood and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.116
H-Index - 105
eISSN - 1545-5017
pISSN - 1545-5009
DOI - 10.1002/pbc.24157
Subject(s) - hereditary spherocytosis , medicine , erythrocyte fragility , spherocytosis , anemia , pediatrics , hemolytic anemia , blood flow , hemolysis , pathology , gastroenterology , splenectomy , spleen
We studied 31 children with hemolytic anemia, or with positive family history for hereditary spherocytosis (HS), to assess the reliability of capillary blood samples for the diagnosis. HS was diagnosed in 20 patients. Cryohemolysis (CH) was positive in 94% and eosin‐5′‐maleimide flow cytometry in 90% of them, whereas flow cytometric osmotic fragility was positive in 94%. Capillary blood sampling showed to be useful for the diagnosis. Simultaneous use of these three tests allows confirming diagnosis in 100% of patients. The use of very small blood volumes (300 µl) allows an earlier diagnosis in neonates and small infants. Pediatr Blood Cancer 2012; 59: 1299–1301. © 2012 Wiley Periodicals, Inc.