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Flow cytometric evaluation of platelet activation in chronic autoimmune thrombocytopenia
Author(s) -
Panzer Simon,
Höcker Lisa,
Vormittag Rainer,
Rieger Miriam,
Koren Daniela,
Dunkler Daniela,
Pabinger Ingrid
Publication year - 2006
Publication title -
pediatric blood and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.116
H-Index - 105
eISSN - 1545-5017
pISSN - 1545-5009
DOI - 10.1002/pbc.21000
Subject(s) - platelet , medicine , p selectin , platelet activation , autoimmune thrombocytopenia , immunology , antibody , gastroenterology , endocrinology
Platelets from 42 patients (platelet counts median 42 × 10 9 /L, range 3–223 × 10 9 /L) with chronic idiopathic autoimmune thrombocytopenia (cAITP) were investigated for P‐Selectin expression and PAC‐1 binding. The results showed that the levels of P‐Selectin positive platelets (n = 20) were higher in cAITP than in controls ( P < 0.0001), and correlated with platelet counts ( P = 0.04). PAC‐1 binding was increased in only six patients, and not correlated with platelet counts. There was no correlation of P‐Selectin or PAC‐1 with detectable platelet antibodies. Thus, platelets are activated in cAITP, but platelets, characterized by PAC‐1 binding, are rare. These are either needed to maintain vascular integrity, or underwent premature sequestration. Pediatr Blood Cancer 2006;47:694–696. © 2006 Wiley‐Liss, Inc.