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Analysis of telomerase activity and RNA expression in a patient with acute promyelocytic leukemia treated with all‐ trans retinoic acid
Author(s) -
Akiyama Masaharu,
Yamada Osamu,
Yanagisawa Takaaki,
Fujisawa Kohji,
Eto Yoshikatsu,
Yamada Hisashi
Publication year - 2006
Publication title -
pediatric blood and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.116
H-Index - 105
eISSN - 1545-5017
pISSN - 1545-5009
DOI - 10.1002/pbc.20392
Subject(s) - acute promyelocytic leukemia , peripheral blood mononuclear cell , retinoic acid , telomerase , cancer research , downregulation and upregulation , medicine , telomerase reverse transcriptase , tretinoin , bone marrow , microbiology and biotechnology , leukemia , in vitro , immunology , biology , gene , biochemistry
In this study, we show that all‐ trans retinoic acid (ATRA) treatment leads to a rapid decrease in telomerase activity, which was associated with the reduction in myeloblasts and occurs before the appearance of myelocytes, in a patient with acute promyelocytic leukemia (APL). Microarray analysis by ATRA treatment for 48 hr in peripheral blood mononuclear cells (in vivo) and in cultured bone marrow mononuclear cells (in vitro) from a patient with APL revealed upregulation of CD11b, CD11c, CCAAT enhancer binding protein ε, Rb1, Mad, and tumor necrosis factor‐related genes; and downregulation of hTERT, c‐Myc, WT1, bcl‐2, and eukaryotic translation elongation factor 1α2. The results might offer the potential to define the molecular mechanism underlying ATRA‐induced granulocytic differentiation in patients with APL, and provide clues to identify novel molecular therapeutic targets. Pediatr Blood Cancer 2006,46:506–511. © 2005 Wiley‐Liss, Inc.