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Rapid and accurate determination of MYCN copy number and 1p deletion in neuroblastoma by quantitative PCR
Author(s) -
Anderson John,
Gibson Sian,
Williamson Dan,
Rampling Dyanne,
Austin Catherine,
Shipley Janet,
Sebire Neil,
Brock Penelope
Publication year - 2006
Publication title -
pediatric blood and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.116
H-Index - 105
eISSN - 1545-5017
pISSN - 1545-5009
DOI - 10.1002/pbc.20311
Subject(s) - neuroblastoma , fluorescence in situ hybridization , medicine , real time polymerase chain reaction , fish <actinopterygii> , biopsy , gene duplication , pathology , cancer research , biology , gene , genetics , fishery , chromosome , cell culture
MYCN amplification and 1p36 deletion are adverse prognostic factors in neuroblastoma, and rapid accurate determination of MYCN amplification is essential for risk stratification. MYCN copy number and 1p36 deletion status were determined by fluorescence in situ hybridization (FISH) and real time PCR in a diagnostic pathology laboratory setting on 35 consecutive patients with neuroblastoma. The PCR technique was technically successful in all cases and results were generally available within 24 hr of biospy. There was no discordance between FISH and PCR results. Real time PCR is a reliable, accurate, and simple technique that can be applied to small neuroblastoma biopsies allowing rapid diagnosis. © 2005 Wiley‐Liss, Inc.