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Invasive adenoviral infection in a recipient of unrelated bone marrow transplantation: Problems with diagnostic PCR
Author(s) -
Ishida Hiroyuki,
ShinNakai Noriko,
Yoshihara Takao,
Teramura Tomoko,
Imamura Toshihiko,
Mukouyama Atsushi,
Morimoto Akira,
Imashuku Shinsaku
Publication year - 2005
Publication title -
pediatric blood and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.116
H-Index - 105
eISSN - 1545-5017
pISSN - 1545-5009
DOI - 10.1002/pbc.20275
Subject(s) - polymerase chain reaction , medicine , virology , cidofovir , transplantation , hematopoietic stem cell transplantation , genome , real time polymerase chain reaction , mastadenovirus , gene , genetic enhancement , virus , biology , adenoviridae , genetics
A hematopoietic stem cell transplantation (SCT) recipient developed severe diarrhea and fever. A rapid test for the presence of adenovirus (AdV)‐specific antigen in the patient's stools was positive; however, AdV genome was not detected by conventional or real‐time polymerase chain reaction (PCR). AdV was confirmed by specific PCR for AdV serotype 7 and by an AdV hexon/fiber gene DNA sequence homology search of the PCR product. We suspect that conventional/real‐time PCR failed to detect AdV due to nine silent single base substitutions in the extracted AdV genome. Treatment with 1 mg/kg cidofovir (CDV) intravenously three times a week was effective. © 2004 Wiley‐Liss, Inc.