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DOR‐1, A novel CD10+ stromal cell line derived from progressive Langerhans cell histiocytosis of bone
Author(s) -
Gogusev Jean,
Telvi Louise,
Murakami Ichiro,
Lepelletier Yves,
Nezelof Christian,
Stojkoski Alexandre,
Glorion Christophe,
Jaubert Francis
Publication year - 2005
Publication title -
pediatric blood and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.116
H-Index - 105
eISSN - 1545-5017
pISSN - 1545-5009
DOI - 10.1002/pbc.20090
Subject(s) - langerhans cell histiocytosis , pathology , immunophenotyping , langerhans cell , cd68 , stromal cell , biology , fluorescence in situ hybridization , microbiology and biotechnology , medicine , immunology , immunohistochemistry , flow cytometry , antigen , genetics , disease , gene , chromosome
Background Langerhans cell histiocytosis (LCH) is granulomatous proliferative disorder characterized by the presence of activated Langerhans cells admixed with macrophages, lymphocytes, and eosinophils. In an effort to obtain an LCH ex vivo model, we succeeded in establishing the DOR‐1 cell line from an LCH lesion of bone in a 3‐year‐old girl. Procedure The DOR‐1 cell line was established from a CD1a immunoreactive LCH lesion of bone maintained in long‐term cell culture. The phenotypic characteristics were assessed by immuno‐cytochemistry and fluorescence activated cell sorter (FACS) analysis. Cytogenetic analysis was performed by RHG‐banding that was supplemented by fluorescence in situ hybridization (FISH). Results The DOR‐1 cells grew in vitro as a poorly differentiated mesenchymal‐like cells with a doubling time between 72 and 96 hr. The cells exhibited pleomorphism and consistent immuno‐reactivity for CD10 (50%), CD13 (55%), CD68 (65%), and CD117 (70%) while CD1a, Langerin and HLA‐DR were not detected. By RHG‐banding, several aberrant chromosomes were detected including the t (9; 17) (p23; p13) translocation and a pair of long dicentric marker chromosomes indicating clonal abnormality. Functionally, exposure to 33 nM 12‐O‐tetradecanoyl phorbol mirystate‐13‐acetate (TPA) induced DOR‐1 cell differentiation with appearance of cytoplasmic extensions. Conclusions The DOR‐1 cell line exhibits distinct immuno‐cytochemical features and carries the t (9; 17) (p23; p13) translocation suggesting involvement of stromal‐like cell lineage in LCH initiation and progression. © 2004 Wiley‐Liss, Inc.