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A model of pulmonary adenocarcinoma in transgenic mice expressing the simian virus 40 T antigen driven by the rat Calbindin‐D9K (CaBP9K) promoter
Author(s) -
ChailleyHeu Bernadette,
Rambaud Caroline,
BarlierMur AnneMarie,
GalateauSalle Françoise,
Perret Christine,
Capron Frédérique,
LacazeMasmonteil Thierry
Publication year - 2001
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.960
Subject(s) - transgene , genetically modified mouse , antigen , biology , simian , virology , adenocarcinoma , pulmonary adenocarcinoma , virus , cancer research , microbiology and biotechnology , gene , immunology , cancer , genetics
Abstract Lung cancer is the most frequent cause of cancer deaths. Its origin and development remain poorly understood, partly because of the lack of pertinent animal models. This study produced transgenic mice expressing the simian virus (SV) 40 T antigen (Tag) driven by a 1011 base‐pair DNA fragment of the rat Calbindin‐D9K (CaBP9K) promoter. All transgenic animals developed multifocal pulmonary tumours with pathological and ultrastructural features consistent with adenocarcinomas. Using immunohistochemistry, northern blot or western blot, tumours were found to express the transcription factor TTF‐1, as well as specific markers of the peripheral airway Clara cells (CC10) and alveolar type II cells (surfactant proteins A, B, C, and D). This model, with its similarities to human adenocarcinoma, should be useful not only for addressing the mechanisms underlying the development and progression of lung cancer, but also for testing new therapeutic approaches. Copyright © 2001 John Wiley & Sons, Ltd.