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Use of laser capture microdissection, cDNA microarrays, and tissue microarrays in advancing our understanding of prostate cancer
Author(s) -
Rubin Mark A.
Publication year - 2001
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.892
Subject(s) - laser capture microdissection , dna microarray , tissue microarray , microdissection , complementary dna , biology , computational biology , prostate cancer , microarray , microbiology and biotechnology , cancer , gene expression , gene , genetics
One difficulty in studying epithelial tumors has been the inability to isolate pure samples for DNA and RNA analysis. Prostate cancer, with its infiltrative nature, is particularly challenging. The Combination of several new technologies should help overcome these hurdles. Laser capture microdissection uses a laser beam to achieve transfer of pure cell populations for isolation of DNA, RNA, and protein. High‐throughput analysis of these samples can be achieved by using cDNA expression microarrays. High‐density tissue microarrays should allow for validation of differentially expressed genes. This review will concentrate on the application of laser capture microdissection, cDNA microarrays, and tissue microarrays in the area of prostate cancer research. Copyright © 2001 John Wiley & Sons, Ltd.