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Dystrophin deficiency impairs vascular structure and function in the canine model of Duchenne muscular dystrophy
Author(s) -
Kodippili Kasun,
Thorne Pamela K,
Laughlin M Harold,
Duan Dongsheng
Publication year - 2021
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.5704
Subject(s) - dystrophin , enos , duchenne muscular dystrophy , medicine , endocrinology , endothelium , muscular dystrophy , vascular smooth muscle , pathogenesis , nitric oxide , mdx mouse , nitric oxide synthase , smooth muscle
Duchenne muscular dystrophy (DMD) is a muscle‐wasting disease caused by dystrophin deficiency. Vascular dysfunction has been suggested as an underlying pathogenic mechanism in DMD. However, this has not been thoroughly studied in a large animal model. Here we investigated structural and functional changes in the vascular smooth muscle and endothelium of the canine DMD model. The expression of dystrophin and endothelial nitric oxide synthase (eNOS), neuronal NOS (nNOS), and the structure and function of the femoral artery from 15 normal and 16 affected adult dogs were evaluated. Full‐length dystrophin was detected in the endothelium and smooth muscle in normal but not affected dog arteries. Normal arteries lacked nNOS but expressed eNOS in the endothelium. NOS activity and eNOS expression were reduced in the endothelium of dystrophic dogs. Dystrophin deficiency resulted in structural remodeling of the artery. In affected dogs, the maximum tension induced by vasoconstrictor phenylephrine and endothelin‐1 was significantly reduced. In addition, acetylcholine‐mediated vasorelaxation was significantly impaired, whereas exogenous nitric oxide‐induced vasorelaxation was significantly enhanced. Our results suggest that dystrophin plays a crucial role in maintaining the structure and function of vascular endothelium and smooth muscle in large mammals. Vascular defects may contribute to DMD pathogenesis. © 2021 The Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.