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Mixed‐species biofilm compromises wound healing by disrupting epidermal barrier function
Author(s) -
Roy Sashwati,
Elgharably Haytham,
Sinha Mithun,
Ganesh Kasturi,
Chaney Sarah,
Mann Ethan,
Miller Christina,
Khanna Savita,
Bergdall Valerie K,
Powell Heather M,
Cook Charles H,
Gordillo Gayle M,
Wozniak Daniel J,
Sen Chandan K
Publication year - 2014
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.4360
Subject(s) - biofilm , wound healing , barrier function , wound closure , transepidermal water loss , microbiology and biotechnology , chronic wound , function (biology) , biology , epidermis (zoology) , medicine , immunology , pathology , bacteria , anatomy , genetics , stratum corneum
Abstract In chronic wounds, biofilm infects host tissue for extended periods of time. This work establishes the first chronic preclinical model of wound biofilm infection aimed at addressing the long‐term host response. Although biofilm‐infected wounds did not show marked differences in wound closure, the repaired skin demonstrated compromised barrier function. This observation is clinically significant, because it leads to the notion that even if a biofilm infected wound is closed, as observed visually, it may be complicated by the presence of failed skin, which is likely to be infected and/or further complicated postclosure. Study of the underlying mechanisms recognized for the first time biofilm‐inducible miR‐146a and miR‐106b in the host skin wound‐edge tissue. These miRs silenced ZO ‐1 and ZO ‐2 to compromise tight junction function, resulting in leaky skin as measured by transepidermal water loss ( TEWL ). Intervention strategies aimed at inhibiting biofilm‐inducible miRNAs may be productive in restoring the barrier function of host skin. Copyright © 2014 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.