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An activation of LC3A ‐mediated autophagy contributes to de novo and acquired resistance to EGFR tyrosine kinase inhibitors in lung adenocarcinoma
Author(s) -
Nihira Kaito,
Miki Yasuhiro,
Iida Shinya,
Narumi Sodai,
Ono Katsuhiko,
Iwabuchi Erina,
Ise Kazue,
Mori Kazushige,
Saito Mikiyoshi,
Ebina Masahito,
Sato Ikuro,
Maemondo Makoto,
YamadaOkabe Hisafumi,
Kondo Takashi,
Sasano Hironobu
Publication year - 2014
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.4354
Subject(s) - gefitinib , autophagy , erlotinib , cancer research , adenocarcinoma , tyrosine kinase , lung cancer , biology , medicine , cancer , oncology , epidermal growth factor receptor , signal transduction , apoptosis , microbiology and biotechnology , biochemistry
The development of therapeutic resistance to EGFR tyrosine kinase inhibitors ( EGFR‐TKIs , ie erlotinib or gefitinib) has been the major clinical problem when treating lung adenocarcinoma patients with these agents. However, its mechanisms have not necessarily been well studied to this date. Autophagy has been recently considered to play pivotal roles in escaping from the effects of anti‐neoplastic agents. Therefore, in this study, we examined its roles in the development of resistance to EGFR‐TKIs in lung adenocarcinoma. We first established erlotinib‐resistant cell lines ( PC9 / ER ) from parental PC9 cells by exposing the cells to erlotinib. In PC9 / ER , autophagy‐related LC3A expression came to be up‐regulated and constitutive activation of LC3A ‐mediated autophagy became more pronounced through the process of acquiring therapeutic resistance. In addition, inhibition of LC3A or autophagy restores sensitivity to EGFR‐TKIs in PC9 / ER . LC3A was also activated at the transcriptional level in de novo resistant cells via demethylation of the MAP1LC3A gene. We then evaluated the status of LC3A in 169 lung adenocarcinoma patients using immunohistochemistry. LC3A immunoreactivity was only detected in carcinoma cells (89/169 patients), not in non‐tumoural cells. In addition, LC3A immunoreactivity was significantly correlated with progression‐free survival ( p = 0.0039) and overall survival ( p = 0.0040) of 35 patients treated with EGFR‐TKIs . The results of our present study demonstrated that LC3A ‐mediated autophagy in carcinoma cells was involved in the development of resistance to EGFR‐TKIs , and that LC3A could serve as a promising therapeutic target for overcoming resistance to EGFR‐TKIs and a novel predictor of response to EGFR‐TKIs in lung adenocarcinoma patients. Copyright © 2014 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd