Premium
Identification of recurrent FGFR3 fusion genes in lung cancer through kinome‐centred RNA sequencing
Author(s) -
Majewski Ian J,
Mittempergher Lorenza,
Davidson Nadia M,
Bosma Astrid,
Willems Stefan M,
Horlings Hugo M,
de Rink Iris,
Greger Liliana,
Hooijer Gerrit KJ,
Peters Dennis,
Nederlof Petra M,
Hofland Ingrid,
de Jong Jeroen,
Wesseling Jelle,
Kluin Roelof JC,
Brugman Wim,
Kerkhoven Ron,
Nieboer Frank,
Roepman Paul,
Broeks Annegien,
Muley Thomas R,
Jassem Jacek,
Niklinski Jacek,
van Zandwijk Nico,
Brazma Alvis,
Oshlack Alicia,
van den Heuvel Michel,
Bernards René
Publication year - 2013
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.4209
Subject(s) - kinome , fusion gene , cancer research , biology , lung cancer , oncogene proteins , computational biology , cancer , gene , pten , bioinformatics , genetics , signal transduction , medicine , oncology , regulation of gene expression , pi3k/akt/mtor pathway
Oncogenic fusion genes that involve kinases have proven to be effective targets for therapy in a wide range of cancers. Unfortunately, the diagnostic approaches required to identify these events are struggling to keep pace with the diverse array of genetic alterations that occur in cancer. Diagnostic screening in solid tumours is particularly challenging, as many fusion genes occur with a low frequency. To overcome these limitations, we developed a capture enrichment strategy to enable high‐throughput transcript sequencing of the human kinome. This approach provides a global overview of kinase fusion events, irrespective of the identity of the fusion partner. To demonstrate the utility of this system, we profiled 100 non‐small cell lung cancers and identified numerous genetic alterations impacting fibroblast growth factor receptor 3 ( FGFR3 ) in lung squamous cell carcinoma and a novel ALK fusion partner in lung adenocarcinoma. © 2013 The Authors. Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.