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An osteosarcoma zebrafish model implicates Mmp‐19 and Ets‐1 as well as reduced host immune response in angiogenesis and migration
Author(s) -
Mohseny Alexander B,
Xiao Wei,
Carvalho Ralph,
Spaink Herman P,
Hogendoorn Pancras CW,
CletonJansen AnneMarie
Publication year - 2012
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.3998
Subject(s) - angiogenesis , mesenchymal stem cell , biology , osteosarcoma , zebrafish , immune system , cancer research , transplantation , immunology , matrix metalloproteinase , progenitor cell , microbiology and biotechnology , gene , stem cell , genetics , medicine
About 40% of osteosarcoma patients die of metastases. Novel strategies to improve treatment of metastatic patients require a better understanding of the processes involved, like angiogenesis, migration, and the immune response. However, the rarity of osteosarcoma and its heterogeneity make this neoplasm difficult to study. Recently we reported malignant transformation of mouse mesenchymal stem cells (MSCs) which formed osteosarcoma upon transplantation into mice. Here we studied these cells in zebrafish embryos and found that transformed MSCs induced angiogenesis and migrated through the bodies of the embryos, but this was never observed with non‐transformed normal MSCs (progenitors of the transformed MSCs). Whole genome expression analysis of both the cells and the host showed that angiogenesis and migration‐related genes matrix metalloproteinase 19 (Mmp‐19) and erythroblastosis virus E26 oncogene homologue 1 (Ets‐1) were overexpressed in transformed MSCs compared to normal MSCs. Investigating the host response, embryos injected with transformed MSCs showed decreased expression of immune response‐related genes, especially major histocompatibility complex class 1 (mhc1ze) , as compared to embryos injected with normal MSCs. These findings contribute to the identification of genetic events involved in angiogenesis, migration, and host response providing targets as well as an appropriate model for high‐throughput drug screens. Copyright © 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.