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Autophagy mediates survival of pancreatic tumour‐initiating cells in a hypoxic microenvironment
Author(s) -
Rausch Vanessa,
Liu Li,
Apel Anja,
Rettig Theresa,
Gladkich Jury,
Labsch Sabrina,
Kallifatidis Georgios,
Kaczorowski Adam,
Groth Ariane,
Gross Wolfgang,
Gebhard Martha M,
Schemmer Peter,
Werner Jens,
Salnikov Alexei V,
Zentgraf Hanswalter,
Büchler Markus W,
Herr Ingrid
Publication year - 2012
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.3994
Subject(s) - autophagy , clonogenic assay , cancer research , pancreatic cancer , cancer stem cell , western blot , biology , population , stem cell , apoptosis , chemistry , microbiology and biotechnology , cancer , medicine , gene , genetics , environmental health
Involvement of dysregulated autophagy in cancer growth and progression has been shown in different tumour entities, including pancreatic ductal adenocarcinoma (PDA). PDA is an extremely aggressive tumour characterized by a small population of highly therapy‐resistant cancer stem cells (CSCs) capable of self‐renewal and migration. We examined whether autophagy might be involved in the survival of CSCs despite nutrition and oxygen deprivation typical for the hypoxic tumour microenvironment of PDA. Immunohistochemistry revealed that markers for hypoxia, CSCs and autophagy are co‐expressed in patient‐derived tissue of PDA. Hypoxia starvation (H/S) enhanced clonogenic survival and migration of established pancreatic cancer cells with stem‐like properties (CSC $^{\rm{high}})$ , while pancreatic tumour cells with fewer stem cell markers (CSC $^{\rm{low}})$ did not survive these conditions. Electron microscopy revealed more advanced autophagic vesicles in CSC $^{\rm{high}}$ cells, which exhibited higher expression of autophagy‐related genes under normoxic conditions and relative to CSC $^{\rm{low}}$ cells, as found by RT‐PCR and western blot analysis. LC3 was already fully converted to the active LC3‐II form in both cell lines, as evaluated by western blot and detection of accumulated GFP‐LC3 protein by fluorescence microscopy. H/S increased formation of autophagic and acid vesicles, as well as expression of autophagy‐related genes, to a higher extent in CSC $^{\rm{high}}$ cells. Modulation of autophagy by inhibitors and activators resensitized CSC $^{\rm{high}}$ to apoptosis and diminished clonogenicity, spheroid formation, expression of CSC‐related genes, migratory activity and tumourigenicity in mice. Our data suggest that enhanced autophagy levels may enable survival of CSC $^{\rm{high}}$ cells under H/S. Interference with autophagy‐activating or ‐inhibiting drugs disturbs the fine‐tuned physiological balance of enhanced autophagy in CSC and switches survival signalling to suicide. Copyright © 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.