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Cystogenic potential of CD133+ progenitor cells of human polycystic kidneys
Author(s) -
Carvalhosa Raquel,
Deambrosis Ilaria,
Carrera Paola,
Pasquino Chiara,
Rigo Francesca,
Ferrari Maurizio,
Lasaponara Fedele,
Ranghino Andrea,
Biancone Luigi,
Segoloni Giuseppe,
Bussolati Benedetta,
Camussi Giovanni
Publication year - 2011
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.2920
Subject(s) - progenitor cell , matrigel , polycystic kidney disease , biology , stem cell , pathology , progenitor , in vitro , phenotype , cellular differentiation , renal stem cell , kidney , microbiology and biotechnology , endocrinology , medicine , gene , biochemistry
In autosomal dominant polycystic kidney disease, cysts arise focally and disrupt normal renal tissue leading to renal failure. In the present study, we show that cyst‐lining cells express the stem cell marker CD133. CD133+ progenitor cells isolated from polycystic kidney, carrying mutations of PKD genes, showed a dedifferentiated phenotype similar to CD133+ progenitor cells from normal kidney. However, these cells were more proliferative and presented a defective epithelial differentiation phenotype with respect to normal renal CD133+ cells as they were not able to express all tubular epithelial cell markers when cultured in epithelial differentiation medium. Polycystic CD133+ cells, in contrast to normal renal CD133+ cells, formed cysts in vitro in a three‐dimensional culture system and in vivo when injected subcutaneously within Matrigel in SCID mice. Rapamycin treatment reduced in vitro proliferation of polycystic CD133+ cells and decreased cystogenesis both in vitro and in vivo. The in vitro epithelial differentiation was only partially improved by rapamycin. These results indicate that polycystic CD133+ cells retain a dedifferentiated phenotype and the ability to generate cysts. Copyright © 2011 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.