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Prominent expression of sialyl Lewis X‐capped core 2‐branched O ‐glycans on high endothelial venule‐like vessels in gastric MALT lymphoma
Author(s) -
Kobayashi Motohiro,
Mitoma Junya,
Hoshino Hitomi,
Yu ShinYi,
Shimojo Yasuyo,
Suzawa Kenichi,
Khoo KayHooi,
Fukuda Minoru,
Nakayama Jun
Publication year - 2011
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.2851
Subject(s) - sialyl lewis x , glycan , lymphoma , biology , microbiology and biotechnology , chemistry , pathology , cancer research , immunology , selectin , cell adhesion molecule , glycoprotein , medicine
High endothelial venule (HEV)‐like vessels have been observed in gastric B cell lymphoma of mucosa‐associated lymphoid tissue type (MALT lymphoma), as well as in its preceding lesion, chronic Helicobacter pylori gastritis. Previously we reported that glycans on HEV‐like vessels in the latter lesion served as L ‐selectin ligands, although their function is unclear. We have investigated sialyl Lewis X (sLeX)‐related glycoepitopes and found that MECA‐79 − /HECA‐452 + /NCC‐ST‐439 + HEV‐like vessels preferentially mark gastric MALT lymphoma compared to chronic H. pylori gastritis. We then constructed CHO cell lines expressing potential MECA‐79 − /HECA‐452 + /NCC‐ST‐439 + glycans, as well as other sLeX‐type glycans, on CD34 and evaluated L ‐selectin binding to those cells, using L ‐selectin–IgM chimera binding and lymphocyte adhesion assays. L ‐selectin–IgM chimeras bound to CHO cells expressing 6‐sulpho‐sLeX attached to core 2‐branched O ‐glycans with or without 6‐sulpho‐sLeX attached to extended core 1 O ‐glycans, but only marginally to other CHO cell lines. By contrast, CHO cells expressing 6‐sulpho‐sLeX attached to extended core 1 and/or core 2‐branched O ‐glycans, as well as non‐sulphated sLeX attached to core 2‐branched O ‐glycans, showed substantial lymphocyte binding, while binding was negligible on lines expressing 6‐sulpho‐ and non‐sulphated sLeX attached to N ‐glycans and non‐sulphated sLeX attached to extended core 1 O ‐glycans. These results indicate that MECA‐79 − /HECA‐452 + /NCC‐ST‐439 + glycans, specifically, 6‐sulpho‐ and non‐sulphated sLeXs attached to core 2‐branched O ‐glycans, expressed on HEV‐like vessels in gastric MALT lymphoma function as L ‐selectin ligands and likely contribute to H. pylori ‐specific T cell recruitment in the progression of gastric MALT lymphoma. Copyright © 2011 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.