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PMEPA1 promotes androgen receptor‐negative prostate cell proliferation through suppressing the Smad3/4–c‐Myc–p21 $^{{\rm Cip1}}$ signaling pathway
Author(s) -
Liu Rong,
Zhou Zhongmei,
Huang Jian,
Chen Ceshi
Publication year - 2011
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.2834
Subject(s) - prostate cancer , biology , cell growth , cancer research , androgen receptor , signal transduction , prostate , cell , microbiology and biotechnology , cell cycle , cancer , genetics
The PMEPA1 gene has been shown to suppress the androgen receptor (AR) and TGFβ signaling pathways and is abnormally expressed in prostate tumours. However, the role and mechanism action of PMEPA1 in AR‐negative prostate cancer are unclear. Here, we demonstrate that inhibition of PMEPA1 suppresses AR‐negative RWPE1 and PC‐3 prostate cell proliferation through up‐regulating the p 21 transcription. Additionally, PMEPA1 overexpression suppresses the p21 expression and promotes cell proliferation. PMEPA1 is induced by TGFβ as a negative feedback loop to suppress Smad3 phosphorylation and nuclear translocation; up‐regulates c‐Myc; down‐regulates p21; and promotes PC‐3 cell proliferation. The PMEPA1 functions depend on its Smad2/3 binding motif. Consistently, depletion of Smad3/4, but not Smad2, blocks PMEPA1's functions of regulating c‐Myc and p21. Importantly, stable depletion of PMEPA1 in PC‐3 inhibits xenograft growth. Finally, we found that PMEPA1 is overexpressed in a subset of prostate cancer cell lines and tumours. These findings suggest that PMEPA1 may promote AR‐negative prostate cancer cell proliferation through p21. Copyright © 2011 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.