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Ectopic expression of the microtubule‐dependent motor protein Eg5 promotes pancreatic tumourigenesis
Author(s) -
Liu Min,
Wang Xiuchao,
Yang Yunfan,
Li Dengwen,
Ren He,
Zhu Qigang,
Chen Qiang,
Han Shuyong,
Hao Jihui,
Zhou Jun
Publication year - 2010
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.2706
Subject(s) - pancreatic cancer , ectopic expression , cancer research , biology , microtubule , polyploid , cancer , cell culture , microbiology and biotechnology , gene , genetics , ploidy
Pancreatic cancer is a highly aggressive disease with a grim prognosis, due to its late diagnosis, propensity to rapidly metastasize, and resistance to therapy. The molecular events underlying this remain poorly defined. Here we report the overexpression and gene copy number gain of the microtubule‐dependent motor protein Eg5 in human pancreatic cancer samples. We also show that Eg5 expression correlates with clinicopathological parameters of pancreatic cancer and promotes anchorage‐independent cell growth and tumour formation in mice. In addition, Eg5 is up‐regulated in pancreatic cancer cell lines and enhances cell proliferation in an ATPase activity‐dependent manner. Our data further reveal that Eg5 overexpression causes the formation of multipolar spindles and multinucleation and induces the accumulation of polyploid cells. These findings demonstrate a role for Eg5 in pancreatic tumourigenesis and indicate a potential for targeting Eg5 in pancreatic cancer treatment. Copyright © 2010 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.