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Glioblastoma stem cells produce vascular endothelial growth factor by activation of a G‐protein coupled formylpeptide receptor FPR
Author(s) -
Yao XH,
Ping YF,
Chen JH,
Xu CP,
Chen DL,
Zhang R,
Wang JM,
Bian XW
Publication year - 2008
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.2356
Subject(s) - glioma , angiogenesis , stem cell , cancer research , biology , receptor , chemotaxis , endothelial stem cell , vascular endothelial growth factor , cell culture , microbiology and biotechnology , neural stem cell , growth factor , vegf receptors , in vitro , genetics
Glioma stem cells (GSCs), or stem cell‐like glioma cells, isolated from malignant glioma cell lines, were capable of producing vascular endothelial growth factor (VEGF). However, the exact role of such tumour cells in angiogenesis remains unknown. In this study, we isolated a small proportion of CD133 + GSCs from the human glioblastoma cell line U87 and found that these GSCs possessed multipotent differentiation potential and released high levels of VEGF as compared with CD133 − tumour cells. The CD133 + GSCs also formed larger xenograft tumours that contained higher VEGF immunoreactivity and denser microvessels. Moreover, GSCs expressed a functional G protein‐coupled formylpeptide receptor FPR, which was activated by a chemotactic peptide ligand, N‐formylmethionyl‐leucyl‐phenylalanine (fMLF), to mediate calcium flux and the production of VEGF by GSCs. Our results indicate that FPR expressed by human GSCs may play an important role in glioma angiogenesis. Copyright © 2008 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.