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cDNA expression profiling of chondrosarcomas: Ollier disease resembles solitary tumours and alteration in genes coding for components of energy metabolism occurs with increasing grade
Author(s) -
Rozeman Leida B,
Hameetman Liesbeth,
van Wezel Tom,
Taminiau Antonie HM,
CletonJansen Anne Marie,
Hogendoorn Pancras CW,
Bovée Judith VMG
Publication year - 2005
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1813
Subject(s) - chondrosarcoma , biology , cartilage , gene , pathology , complementary dna , immunohistochemistry , gene expression profiling , cancer research , gene expression , genetics , medicine , anatomy
Abstract Conventional central chondrosarcomas are malignant cartilaginous tumours, occasionally arising secondary to either solitary or multiple (Ollier disease) enchondromas. Recurrences may have progressed in grade. The aims of the present study were to identify putative differences in gene expression between solitary and Ollier disease‐related tumours, and to elucidate signalling pathways involved in tumour progression by genome‐wide cDNA expression analysis. Arrays enriched for cartilage‐specific cDNAs and genes involved in general tumourigenesis were used to analyse enchondromas ( n = 3, two with Ollier disease), chondrosarcomas of different grades ( n = 19, three with Ollier disease), normal resting‐zone cartilage ( n = 2), and chondrosarcoma cells in culture ( n = 7). The arrays were analysed by unsupervised hierarchical clustering, significant analysis of microarray, and T ‐tests. Confirmation of data was performed by immunohistochemistry and quantitative reverse transcriptase polymerase chain reaction (RT‐PCR). Ollier disease cases and solitary tumours revealed similar expression profiles, suggesting that the same signalling pathways are involved in tumourigenesis. Interestingly, JunB protein expression was significantly higher in grade I chondrosarcomas than in enchondromas ( p = 0.009), which could be of diagnostic relevance. Upon chondrosarcoma progression, matrix‐associated genes are down‐regulated, reflecting the histology of high‐grade tumours. An increase in glycolysis‐associated, and a decrease in oxidative phosphorylation‐related, genes was found in high‐grade tumours. These findings suggest an adaptation in energy supply upon progression towards higher grade. Copyright © 2005 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.