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An animal model of SARS produced by infection of Macaca mulatta with SARS coronavirus
Author(s) -
Qin Chuan,
Wang Jianwei,
Wei Qiang,
She Mingpeng,
Marasco Wayne A,
Jiang Hong,
Tu Xinming,
Zhu Hua,
Ren Lili,
Gao Hong,
Guo Li,
Huang Lan,
Yang Renquan,
Cong Zhe,
Guo Lan,
Wang Yanbin,
Liu Yali,
Sun Yili,
Duan Shumin,
Qu Jianguo,
Chen Liangbiao,
Tong Wei,
Ruan Li,
Liu Peimao,
Zhang Hua,
Zhang Jianmin,
Zhang Huiyuan,
Liu Depei,
Liu Qian,
Hong Tao,
He Wei
Publication year - 2005
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1769
Subject(s) - virology , virus , coronavirus , inoculation , pneumonia , pathological , animal model , biology , covid-19 , lung , medicine , pathology , immunology , disease , infectious disease (medical specialty) , endocrinology
A new SARS animal model was established by inoculating SARS coronavirus (SARS‐CoV) into rhesus macaques ( Macaca mulatta ) through the nasal cavity. Pathological pulmonary changes were successively detected on days 5–60 after virus inoculation. All eight animals showed a transient fever 2–3 days after inoculation. Immunological, molecular biological, and pathological studies support the establishment of this SARS animal model. Firstly, SARS‐CoV‐specific IgGs were detected in the sera of macaques from 11 to 60 days after inoculation. Secondly, SARS‐CoV RNA could be detected in pharyngeal swab samples using nested RT‐PCR in all infected animals from 5 days after virus inoculation. Finally, histopathological changes of interstitial pneumonia were found in the lungs during the 60 days after viral inoculation: these changes were less marked at later time points, indicating that an active healing process together with resolution of an acute inflammatory response was taking place in these animals. This animal model should provide insight into the mechanisms of SARS‐CoV‐related pulmonary disease and greatly facilitate the development of vaccines and therapeutics against SARS. Copyright © 2005 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.