Premium
Proteomic analysis of differential protein expression in human atherosclerotic plaque progression
Author(s) -
Donners Marjo MPC,
Verluyten Monique J,
Bouwman Freek G,
Mariman Edwin CM,
Devreese Bart,
Vanrobaeys Frank,
van Beeumen Jozef,
van den Akker Luc HJM,
Daemen Mat JAP,
Heeneman Sylvia
Publication year - 2005
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1749
Subject(s) - blot , immunohistochemistry , thrombus , gene isoform , pathology , microbiology and biotechnology , differential diagnosis , western blot , pathological , biology , medicine , biochemistry , gene
Abstract In this study, differential protein expression was assessed during human atherosclerotic plaque progression. A multifaceted approach was used in which differential protein expression was studied by two‐dimensional (2D) gel electrophoresis and validated in individual patients using western blotting and immunohistochemistry. 2D profiles of whole‐mount advanced stable lesions were compared to those of plaques containing a thrombus. Mass spectrometry analysis identified vinexin‐β and α 1 ‐antitrypsin (AAT) in the same spot that was differentially expressed in plaques with a thrombus. Immunohistochemistry and western blotting showed limited expression of both vinexin‐β and AAT in early lesions, whereas high expression of both proteins was found in advanced lesions. Differential expression of vinexin‐β in lesions with a thrombus compared to stable plaques could not be confirmed, indicating the importance of validation of proteomic analysis. For AAT, western blotting of 2D gels revealed expression of six isoforms in advanced plaques, one of which was confirmed to be solely expressed in thrombus‐containing plaques. In conclusion, vinexin‐β is expressed in advanced human atherosclerotic plaques, but differential expression of this protein in lesions with a thrombus versus stable plaques could not be confirmed. However, this analysis revealed expression of six isoforms of AAT in advanced plaques, one of which was uniquely expressed in thrombus‐containing plaques. Copyright © 2005 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.