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Determination of transforming growth factor beta 1 mRNA expression in breast carcinomas by in situ hybridization
Author(s) -
Walker Rosemary A.,
Gallacher Barbara
Publication year - 1995
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711770204
Subject(s) - in situ hybridization , transforming growth factor beta , stromal cell , messenger rna , biology , microbiology and biotechnology , beta (programming language) , gene expression , tgf beta 1 , cancer research , transforming growth factor , pathology , endocrinology , gene , medicine , biochemistry , computer science , programming language
The expression of transforming growth factor beta 1 (TGF‐β 1 ) mRNA has been determined in 16 breast carcinomas using in situ hybridization and compared with TGF‐β protein as detected by antibodies against TGF‐βT 1 and TGF‐β 1 plus TGF‐β 2 . Digoxigenin‐labelled riboprobes were used, with alkaline phosphatase and immunogold silver detection systems. TGF‐β 1 mRNA was only detected in carcinomas in which TGF‐β 1 protein was found (9 of 16 cases) and not in those with prominent reactivity for TGF‐β 2 . RNA preservation was poor in two other cases in which TGF‐β 1 protein had been detected. In general, those tumours with greater numbers of cells labelled for TGF‐β 1 mRNA had prominent reactivity for TGF‐β 1 protein. The mRNA was localized to cancer cells with no labelling of stromal cells, although in a small number of cases scanty staining for TGF‐β 1 protein had been observed in stromal cells. The incidence of detection of TGF‐β 1 mRNA is lower than the published data from Northern analysis studies of breast carcinomas, suggesting that only higher levels of TGF‐β 1 mRNA expression are being detected by in situ hybridization. However, this approach has provided useful information about the cellular sites of expression of TGF‐β 1 in breast carcinomas.