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Artefacts in electron microscopy: Ultrastructural features of chrysiasis
Author(s) -
Culora G. A.,
Barnett N.,
Theaker J. M.
Publication year - 1995
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711760414
Subject(s) - uranyl acetate , osmium , uranyl , ultrastructure , electron microscope , chemistry , microanalysis , staining , electron probe microanalysis , electron microprobe , mineralogy , anatomy , pathology , biology , biochemistry , physics , optics , ruthenium , medicine , ion , organic chemistry , catalysis
Eleven cases of chrysiasis have been studied ultrastructurally and by electron probe microanalysis. Tissue samples were examined both with and without osmium and urany1 acetate staining. There was a significant morphological difference in the appearances of the gold deposits within aurosomes between the two groups. The untreated group showed finely granular deposits, often arranged in a linear fashion. Aurosomes from samples treated with osmium and urany1 acetate showed more electron‐dense deposits and star‐like formations. We propose that treatment of the tissue with osmium and urany1 acetate causes a change in the electron microscopic appearance of aurosomes, including the formation of the characteristic star‐like aurosomes.