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Absence of in situ hybridization evidence for latent ‐ or lytic‐phase Epstein‐Barr virus infection of preinvasive squamous lesions of the cervix
Author(s) -
Payne S.,
Kernohan N. M.,
Walker F.
Publication year - 1995
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711760303
Subject(s) - lytic cycle , in situ hybridization , epstein–barr virus , carcinogenesis , virus , cervix , polymerase chain reaction , biology , hpv infection , herpesviridae , pathology , virology , viral disease , medicine , messenger rna , gene , cervical cancer , cancer , genetics
To investigate whether Epstein‐Barr virus (EBV) infection of the uterine cervix plays a significant role in cervical carcinogenesis, 30 preinvasive squamous lesions were subjected to in situ hybridization for (EBER−1,−2, and BHLF1) EBV transcripts which are expressed in latent and lytic infection, respectively. Twenty cases were known to contain EBV sequences by previous polymerase chain reaction (PCR) analysis. Irrespective of EBV PCR status or histological grade, none of the 30 cases demonstrated EBV transcripts in squamous epithelial cells. Two cases showed very occasional EBER‐positive stromal cells, most probably representing resident cervical lymphocytes. These findings suggest that EBV plays no part in early cervical carcinogenesis.