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Identification of tumour‐specific translocations in archival material
Author(s) -
Williams G. H.,
Williams E. D.
Publication year - 1995
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711750305
Subject(s) - chromosomal translocation , reverse transcriptase , identification (biology) , nested polymerase chain reaction , polymerase chain reaction , pathology , biology , computational biology , medicine , genetics , gene , botany
A reverse transcriptase nested polymerase reaction (RT‐nPCR) method has been developed to detect the presence of a tumour‐specific translocation in archival formalin‐fixed, paraffin‐embedded tissue. This technique can be applied to the large numbers of tumours in pathology departments' archives, allowing correlation of translocations with morphological diagnosis, response to therapy, and survival.