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Loss of heterozygosity in ductal carcinoma in situ of the breast
Author(s) -
Stratton Michael R.,
Collins Nadine,
Lakhani Sunil R.,
Sloane John P.
Publication year - 1995
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711750207
Subject(s) - loss of heterozygosity , locus (genetics) , ductal carcinoma , biology , breast cancer , allele , in situ , polymerase chain reaction , carcinoma in situ , microsatellite , carcinoma , pathology , cancer , genetics , medicine , gene , chemistry , organic chemistry
Loss of heterozygosity (LOH) at loci reported to show allele loss in invasive breast cancers was examined in ductal in situ carcinomas of the breast using polymorphic short tandem repeats and the polymerase chain reaction (PCR). LOH was detected at all loci examined in at least 11 per cent of the samples examined. The proportion of cases of in situ carcinoma showing LOH at these loci was similar to that previously reported in invasive cancers. Cases of pure in situ cancer without an invasive component exhibited an overall lower frequency of allele loss. LOH at more than one locus was observed in some intraductal cancers. In a small number of cases, LOH was present in the invasive but not in the intraductal component of the tumour, suggesting that mutation at the locus concerned was associated with development of invasive behaviour.