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Demonstration of URR‐duplication variants of human papillomavirus type 6 in paraffin‐embedded tissue sections of one condyloma acuminatum and one buschke‐loewenstein tumour
Author(s) -
Albert Rüubben,
Spelten Bettina,
Albrecht Jörg,
GrußendorfConen ElkeIngrid
Publication year - 1994
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711740103
Subject(s) - gene duplication , condyloma acuminatum , polymerase chain reaction , dna , biology , human papillomavirus , microbiology and biotechnology , gene , medicine , genetics
Human papillomavirus type 6 (HPV 6) induces condylomata acuminata and laryngeal papillomas. Occasionally, HPV 6 may also be found in low‐grade verrucous carcinomas. In some tumours, genetic analysis revealed the presence of HPV 6 variants with rearrangements, mostly DNA duplications, within the upstream regulatory region (URR). In this study, we analysed 98 formalin‐fixed, paraffin‐embedded condylomata acuminata obtained from 54 patients for the presence of URR‐duplication variants of HPV 6. HPV 6 DNA could be amplified by polymerase chain reaction (PCR) from 40 samples. One condyloma acuminatum contained a HPV 6 genome with rearranged URR. Analysis using restriction enzyme cleavage suggested a DNA duplication within the URR of approximately 200 bp, spanning the Hpa II site at nt 7863 and the Dde I site at nt 7843 but not involving the Rsa I site at nt 7633. In addition, we analysed the distribution of the already characterized URR‐duplication variant HPV 6 ACIB within different paraffin‐embedded tissue sections of a Buschke‐Loewenstein tumour. No correlation could be demonstrated between the presence of the rearranged genome and malignant histological changes. This result and the demonstration of an URR‐duplication variant in a typical condyloma acuminatum suggest that duplications within the URR of HPV 6 are not directly related to malignant progression of HPV 6‐induced tumours.