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Production of two monoclonal antibodies (FB1 AND FB21) useful for the identification of human B lymphocytes in formalin‐fixed, paraffin‐embedded tissues
Author(s) -
Nozawa Yoshihiro,
Abe Masafumi,
Ohno Hitoshi,
Fukuhara Shirou,
Wakasa Haruki
Publication year - 1994
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711730410
Subject(s) - antigen , monoclonal antibody , biology , microbiology and biotechnology , cd20 , antibody , reed–sternberg cell , epitope , b cell , germinal center , lymphoma , pathology , immunology , medicine , hodgkin lymphoma
Abstract Two monoclonal antibodies (FBI and FB21) reactive in formalin‐fixed, paraffin‐embedded tissue sections are reported in this paper. FB1 and FB21 recognize a cytoplasmic antigen and a surface antigen of B cells, respectively. FBI reacts with mantle zone (MZ) B cells, germinal centre (GC) cells, and marginal zone (MrZ) B cells, but not with T cells in lymphoid tissues. FB21 reacts with MZ B cells, GC cells in lymphoid tissues, and T cells of peripheral blood, but not with MrZ B cells in the spleen. Neither monoclonal antibody (MoAb) reacts with monocytes, granulocytes, or plasma cells. FB1 reacted with all the B‐cell lymphomas tested and with CD20‐positive Reed‐Sternberg cells in two of five cases of Hodgkin's disease, but not with multiple myelomas or T‐cell lymphomas. FB21 reacted with B‐cell lymphoma in 20 of 22 cases, but not with multiple myelomas, T‐cell lymphomas, or Reed‐Sternberg cells of Hodgkin's disease. Immunoprecipitation studies revealed that FB1 recognizes the same two polypeptide chains that are recognized by L26 and is a member of the CD20 antibody cluster. FB21 was thought to recognize a sialic acid‐dependent carbohydrate epitope and this was confirmed at the Fifth International Conference on Human Leukocyte Differentiation Antigens (Boston, 1993) FB21 did not react with splenic MrZ B cells and was different from the pan B markers reported previously [CD20 (L26), CD45RA (MB1), and CD74 (LN‐2)]. FB21 recognizes a subset of B cells and appears to be closely related to CD75/76 antibodies. FB1 and FB21 are useful MoAbs for the diagnosis and analysis of B‐cell lymphomas.