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Production of two novel monoclonal antibodies against human renal glomeruli and theri application to the immunohistochemical investigation of crescentic glomerulonephritis
Author(s) -
Kohda Yukimasa,
Takeya Motohiro,
Arima Seiya,
Arima Seiya,
Nakayama Mahito,
Sato Tatsuo,
Takahashi Kiyoshi
Publication year - 1994
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711720109
Subject(s) - antigen , pathology , glomerular basement membrane , lamina densa , immunohistochemistry , monoclonal antibody , glomerulonephritis , renal glomerulus , immunoelectron microscopy , kidney , basement membrane , antibody , glomerular mesangium , biology , immunology , medicine , endocrinology
Two monoclonal antiboides (MAbs) against human renal glomeruli, HEP1 and HBM1, were produced using isolated human glomeruli as an antigen. Immunohistochemical and immunoelectron microscopic analyses revealed that the recognition site of HEP1 is the cell surface of the visceral glomerularepithelial cells. HEP1 showed no reaction in the renal interstitium or in other tissues. HBM1 recognized an antigen present in the basal lamina of the renal glomeruli, Bowman's capsule, and partly in the basement membrane of renal tubules, but it was not reactive with the mesangial matrix or renal interstitium. The isotype of both MAbs was IgG1, k . The molecular weights of their corresponding antigens were 127–177 and 220kD, respectively. Besides human tissues, HEP1 also showed cross‐reactions with antigens in the visceral glomerular epithelial cells of various animals other than mice. HBM1 recognized human antigen only. Using both MAbs, eight cases of crescentic glomerulonephritis were examined immunohistochemically. As a result, it was demonstrated that the crescents may not be formed by visceral glomerular epithelial cells. From this study, these two MAbs appear to be userful markers for the evaluation of renal glomerular disorders.